Antibody Production and Purification from Creative Biomart

Offered by Creative Biomart, www.creativebiomart.net, a full-servie US biotech company.

 

Definition

An antibody (Ab), also known as an immunoglobulin (Ig), is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target, called an antigen. This molecular recognition by the immune system results in selective production of antibodies that are able to bind the specific antigen. Antibody circulates throughout the blood and lymph where they bind to their specific antigen, enabling it to be cleared from circulation.

Antibody Production

In the broad sense, antibody production refers to the entire process of creating a usable specific antibody, including steps of immunogen preparation, immunization, hybridoma creation, collection, screening, isotyping, purification, and labeling for direct use in a particular method. In the more restricted sense, antibody production refers to the steps leading up to antibody generation but does not include various forms of purifying and labeling the antibody for particular uses.

Antibody production involves preparation of antigen samples and their safe injection into laboratory or farm animals so as to evoke high expression levels of antigen-specific antibodies in the serum, which can then be recovered from the animal. Polyclonal antibodies are recovered directly from serum (bleeds). Monoclonal antibodies are produced by fusing antibody-secreting spleen cells from immunized mice with immortal myeloma cell to create monoclonal hybridoma cell lines that express the specific antibody in cell culture supernatant.

Successful antibody production depends upon careful planning and implementation with respect to several important steps and considerations.

Antibody Purification

Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid or culture supernatant of a hybridoma cell line (monoclonal antibody). Purification methods range from very crude to highly specific.

With more than thousands of monoclonal, polyclonal and diagnostic antibodies and antigens on offer, Creative Biomart is one of the largest suppliers to the clinical research, molecular diagnostics and biopharmaceutical drug development industries. It guarantees the quality and price to be the most cost-effective as a result of its profound expertise and rich experience as well as good reputation.

 

About Creative Biomart

Creative Biomart is a global full-service biotechnology company with profound expertise and experience in recombinant proteins, antibodies and antigens, diagnostic enzymes and pharmaceutical enzymes, Elisa Kits, biosimilar cell lines, transfected stable cell lines etc. to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development. Creative Biomart also has established itself as a world leader in the area of diagnostic antibody generation; using our genetic immunization technology. Our proprietary technology allows the production and identification of highly specific antibodies.

We have an impressive list of international customers and cooperation partners. Our products can be custom ordered or bulk packaged to fit customer’s research needs.

Our Products

We are offering more than 1,000 recombinant proteins, peptides and antibodies. All the products are rigorously tested to meet the most demanding research needs. At the same time, lowest prices in the industry are always guaranteed, such as recombinant proteins,monoclonal antibodies, polyclonal antibodies, recombinant antibodies, diagnostic antibodies, therapeutic antibodies and antigens, diagnostic enzymes, industrial enzymes and pharmaceutical enzymes, Elisa Kits, biosimilar cell lines, transfected stable cell lines, hybridoma etc.

Our Services

Creative Biomart provides full services, such as Protein Expression (Bacterial Expression Systems (E. coli / Bacillus), Yeast Expression Systems (P pastoris / S cerevisiae), Baculovirus-insect Cell Expression Systems, Mammalian Expression Systems (CHO / 293)), Membrane Protein (Custom Membrane Protein Production, Cell Line Development and Membrane Preparation, Custom Lipoparticle Containing Membrane Protein, Lipid-based Protein Immobilization), PEGylation Services, Directed Evolution, Protein Engineering, Mutagenesis Services (Site-Directed Mutagenesis, Site-Saturation Mutagenesis, Controlled Randomization Service), Protein Microarray , Custom cDNA lone etc.